[HTML][HTML] Mapping of glucocorticoid receptor DNA binding domain surfaces contributing to transrepression of NF-κB and induction of apoptosis
Y Tao, C Williams-Skipp, RI Scheinman - Journal of Biological Chemistry, 2001 - ASBMB
Glucocorticoids (GCs) function, in part, through the ability of the glucocorticoid receptor (GR)
to activate gene expression and in part through the transrepression of AP-1 and NF-κB. Here
we characterize the effect of GR DNA binding domain (DBD) mutations, previously analyzed
for changes in the ability to activate gene expression or transrepress AP-1. We have
identified a GR mutant capable of distinguishing between transrepression of NF-κB and AP-
1. Using circular dichroism spectroscopy, we show that this mutation does not appreciably …
to activate gene expression and in part through the transrepression of AP-1 and NF-κB. Here
we characterize the effect of GR DNA binding domain (DBD) mutations, previously analyzed
for changes in the ability to activate gene expression or transrepress AP-1. We have
identified a GR mutant capable of distinguishing between transrepression of NF-κB and AP-
1. Using circular dichroism spectroscopy, we show that this mutation does not appreciably …