A new approach to isolation and culture of human Kupffer cells

EB Alabraba, SM Curbishley, WK Lai… - Journal of …, 2007 - Elsevier
Macrophages are a diverse population of cells that are able to adapt to specific tissue
environments. Kupffer cells are liver resident macrophages and form the largest population
of fixed tissue macrophages. Their isolation offers an exciting opportunity to study this
subpopulation of uniquely adapted cells. However existing Kupffer cell isolation techniques
are tedious and are still largely based on enzymatic digestion to liberate tissue
macrophages from the closely associated surrounding tissue. Isolation techniques have …