[CITATION][C] The role of red cells in haemostasis: the relation between haematocrit, bleeding time and platelet adhesiveness

AJ Hellem, CHF Borchgrevink… - British Journal of …, 1961 - Wiley Online Library
AJ Hellem, CHF Borchgrevink, SB Ames
British Journal of Haematology, 1961Wiley Online Library
METHODS Adhesiveness ofplatelets in vitro was measured by the method of Hellem (1960).
Briefly the method is as follows: Citrated blood is filtered with constant speed through a
plastic tube containing exactly 5 g. of glass beads with a diameter of 0.5 mm. The contact
time is 30 seconds. The difference in the number of platelets before and after filtration is the
number of adhesive platelets. The normal value is 92,000 platelets per cu. mm. with a range
of 52,000-r57, ooo. Expressed in per cent of the total platelet count, thc mean is 42 and the …
METHODS
Adhesiveness ofplatelets in vitro was measured by the method of Hellem (1960). Briefly the method is as follows: Citrated blood is filtered with constant speed through a plastic tube containing exactly 5 g. of glass beads with a diameter of 0.5 mm. The contact time is 30 seconds. The difference in the number of platelets before and after filtration is the number of adhesive platelets. The normal value is 92,000 platelets per cu. mm. with a range of 52,000-r57, ooo. Expressed in per cent of the total platelet count, thc mean is 42 and the range 26-68. In patients with abnormal haematocrit values the amount of citrate was adjusted in order to have a constant citrate concentration in all plasmas. When the haematocrit was 45 per cent, t ml. of 3.13 per cent trisodium citrate dihydrate was added to 9 ml. of blood.
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