Polymorphism in the P2X7 receptor gene and survival in chronic lymphocytic leukaemia

U Thunberg, G Tobin, A Johnson, O Söderberg… - The Lancet, 2002 - thelancet.com
U Thunberg, G Tobin, A Johnson, O Söderberg, L Padyukov, M Hultdin, L Klareskog
The Lancet, 2002thelancet.com
Summary Background The P2X7 receptor is a ligand-gated cation channel that mediates
ATP-induced apoptotic death in haemopoietic and chronic lymphocytic leukaemia (CLL)
cells. We aimed to investigate the clinical effect of a single nucleotide polymorphism in the
P2X7 receptor gene (1513A⇒ C) and correlate findings with the immunoglobulin heavy
chain variable (VH) gene mutation status, a prognostic marker in CLL. Methods We
investigated tumour DMA in 170 patients with CLL using PCR-RFLP analysis with Hhal …
Background
The P2X7 receptor is a ligand-gated cation channel that mediates ATP-induced apoptotic death in haemopoietic and chronic lymphocytic leukaemia (CLL) cells. We aimed to investigate the clinical effect of a single nucleotide polymorphism in the P2X7 receptor gene (1513A⇒C) and correlate findings with the immunoglobulin heavy chain variable (VH) gene mutation status, a prognostic marker in CLL.
Methods
We investigated tumour DMA in 170 patients with CLL using PCR-RFLP analysis with Hhal restriction enzyme cleavage to screen for the polymorphism in the P2X7 receptor gene. The VH gene mutation status was assessed in 165 patients by PCR amplification and nucleotide sequencing. We correlated the findings of the P2X7 receptor genotype with the VH gene mutation data and overall survival and screened for the P2X7 receptor polymorphism in 200 healthy controls.
Findings
Of the 170 patients, 35 (21%) were heterozygous and one (1%) homozygous for the 1513C allele; whereas 134 (79%) had the 1513A/A genotype of the P2X7 receptor gene. Overall survival was significantly longer for patients with CLL heterozygous for the 1513C allele than those with the 1513A/A genotype. Median survival for heterozygous patients was 104 months (range 33–467) and 72 months (1–190) for homozygous patients (p=0·009). Of the 165 patients with CLL in whom we assessed the VH gene mutation status, the VH genes were mutated in 71 (43%) patients and unmutated in 94; 18 (25%) of the 71 patients with mutated genes had 1513C allele compared with 17 (18%) of 94 who had unmutated genes. In patients with mutated VH genes, those with CLL who were 1513C positive had 53 months' longer median survival than did those with the 1513A/A genotype (151 vs 98 months, p=0·011).
Interpretation
The P2X7 polymorphism could affect clinical outcome in CLL, especially in patients with mutated VH genes. Studies are necessary to elucidate the biological role of the P2X7 polymorphism in CLL in vivo.
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