Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations
B Kalyanaraman, V Darley-Usmar, KJA Davies… - Free radical biology and …, 2012 - Elsevier
Free radical biology and medicine, 2012•Elsevier
The purpose of this position paper is to present a critical analysis of the challenges and
limitations of the most widely used fluorescent probes for detecting and measuring reactive
oxygen and nitrogen species. Where feasible, we have made recommendations for the use
of alternate probes and appropriate analytical techniques that measure the specific products
formed from the reactions between fluorescent probes and reactive oxygen and nitrogen
species. We have proposed guidelines that will help present and future researchers with …
limitations of the most widely used fluorescent probes for detecting and measuring reactive
oxygen and nitrogen species. Where feasible, we have made recommendations for the use
of alternate probes and appropriate analytical techniques that measure the specific products
formed from the reactions between fluorescent probes and reactive oxygen and nitrogen
species. We have proposed guidelines that will help present and future researchers with …
The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results.
Elsevier