A simple spectrophotometric method for the quantification of residual haemoglobin in platelet concentrates
P Cookson, J Sutherland, R Cardigan - Vox Sanguinis, 2004 - Wiley Online Library
P Cookson, J Sutherland, R Cardigan
Vox Sanguinis, 2004•Wiley Online LibraryBackground and Objectives High levels of residual haemoglobin (Hb 0· 1 g/l) are known to
decrease the efficiency of pathogen‐inactivation systems. We evaluated three separate
methods to quantify Hb in platelet concentrates (PC). Materials and Methods Nine PC
prepared in platelet additive solution (PASIII)(median platelet yield of 283× 109/unit, range
46–353) were spiked to known Hb concentrations with whole blood and the samples were
measured by using each of three methods: the 3, 3′, 5, 5′‐tetramethylbenzidine (TMB) …
decrease the efficiency of pathogen‐inactivation systems. We evaluated three separate
methods to quantify Hb in platelet concentrates (PC). Materials and Methods Nine PC
prepared in platelet additive solution (PASIII)(median platelet yield of 283× 109/unit, range
46–353) were spiked to known Hb concentrations with whole blood and the samples were
measured by using each of three methods: the 3, 3′, 5, 5′‐tetramethylbenzidine (TMB) …
Background and Objectives High levels of residual haemoglobin (Hb 0·1 g/l) are known to decrease the efficiency of pathogen‐inactivation systems. We evaluated three separate methods to quantify Hb in platelet concentrates (PC).
Materials and Methods Nine PC prepared in platelet additive solution (PASIII) (median platelet yield of 283 × 109/unit, range 46–353) were spiked to known Hb concentrations with whole blood and the samples were measured by using each of three methods: the 3,3′,5,5′‐tetramethylbenzidine (TMB) oxidation method (Sigma Diagnostics, 527‐A); the Harboe spectrophotometric method; and the HemoCue plasma low‐Hb photometer (PLHP).
Results The TMB and Harboe methods showed linear results compared to expected Hb (r2 ≥ 0·981, P < 0·001) over the range tested (0·09–0·28 g/l) when the samples were haemolysed. The TMB method underestimated by an average of 6%, at and around 0·1 g/l Hb, compared to a 4% overestimation by the Harboe method and a threefold overestimation by the PLHP. The Harboe intra‐assay coefficient of variation was ≤ 1·85% across all concentrations, which contrasted with 30% at and around 0·1 g/l for the TMB method.
Conclusions The Harboe spectrophotometric method is convenient, safe, accurate and reproducible, and outperforms the TMB and PLHP methods for quantification of residual Hb in PC.
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