A simple ABO genotyping by PCR using sequence-specific primers with mismatched nucleotides

T Taki, K Kibayashi - Legal Medicine, 2014 - Elsevier
In forensics, the specific ABO blood group is often determined by analyzing the ABO gene.
Among various methods used, PCR employing sequence-specific primers (PCR-SSP) is
simpler than other methods for ABO typing. When performing the PCR-SSP, the pseudo-
positive signals often lead to errors in ABO typing. We introduced mismatched nucleotides at
the second and the third positions from the 3′-end of the primers for the PCR-SSP method
and examined whether reliable typing could be achieved by suppressing pseudo-positive …