[HTML][HTML] Critical role for polar residues in coupling leukotriene B4 binding to signal transduction in BLT1
S Basu, VR Jala, S Mathis, ST Rajagopal… - Journal of biological …, 2007 - ASBMB
Leukotriene B 4 (LTB 4) mediates a variety of inflammatory diseases such as asthma,
arthritis, atherosclerosis, and cancer through activation of the G-protein-coupled receptor,
BLT1. Using in silico molecular dynamics simulations combined with site-directed
mutagenesis we characterized the ligand binding site and activation mechanism for BLT1.
Mutation of residues predicted as potential ligand contact points in transmembrane domains
(TMs) III (H94A and Y102A), V (E185A), and VI (N241A) resulted in reduced binding affinity …
arthritis, atherosclerosis, and cancer through activation of the G-protein-coupled receptor,
BLT1. Using in silico molecular dynamics simulations combined with site-directed
mutagenesis we characterized the ligand binding site and activation mechanism for BLT1.
Mutation of residues predicted as potential ligand contact points in transmembrane domains
(TMs) III (H94A and Y102A), V (E185A), and VI (N241A) resulted in reduced binding affinity …