The uses and limitations of the analysis of cellular phosphoinositides by lipidomic and imaging methodologies
MJO Wakelam - Biochimica et Biophysica Acta (BBA)-Molecular and …, 2014 - Elsevier
Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2014•Elsevier
The advent of mass spectrometric methods has facilitated the determination of multiple
molecular species of cellular lipid classes including the polyphosphoinositides, though to
date methods to analyse and quantify each of the individual three PtdInsP and three PtdInsP
2 species are lacking. The use of imaging methods has allowed intracellular localization of
the phosphoinositide classes but this methodology does not determine the acyl structures.
The range of molecular species suggests a greater complexity in polyphosphoinositide …
molecular species of cellular lipid classes including the polyphosphoinositides, though to
date methods to analyse and quantify each of the individual three PtdInsP and three PtdInsP
2 species are lacking. The use of imaging methods has allowed intracellular localization of
the phosphoinositide classes but this methodology does not determine the acyl structures.
The range of molecular species suggests a greater complexity in polyphosphoinositide …
Abstract
The advent of mass spectrometric methods has facilitated the determination of multiple molecular species of cellular lipid classes including the polyphosphoinositides, though to date methods to analyse and quantify each of the individual three PtdInsP and three PtdInsP2 species are lacking. The use of imaging methods has allowed intracellular localization of the phosphoinositide classes but this methodology does not determine the acyl structures. The range of molecular species suggests a greater complexity in polyphosphoinositide signaling than yet defined but elucidating this will require further method development to be achieved. This article is part of a Special Issue entitled Tools to study lipid functions.
Elsevier