[HTML][HTML] TCR sequencing of single cells reactive to DQ2. 5-glia-α2 and DQ2. 5-glia-ω2 reveals clonal expansion and epitope-specific V-gene usage

S Dahal-Koirala, LF Risnes, A Christophersen… - Mucosal …, 2016 - Elsevier
CD4+ T cells recognizing dietary gluten epitopes in the context of disease-associated
human leukocyte antigen (HLA)-DQ2 or HLA-DQ8 molecules are the key players in celiac
disease pathogenesis. Here, we conducted a large-scale single-cell paired T-cell receptor
(TCR) sequencing study to characterize the TCR repertoire for two homologous
immunodominant gluten epitopes, DQ2. 5-glia-α2 and DQ2. 5-glia-ω2, in blood of celiac
disease patients after oral gluten challenge. Despite sequence similarity of the epitopes, the …