[HTML][HTML] Serine 209, Not Serine 53, Is the Major Site of Phosphorylation in Initiation Factor eIF-4E in Serum-treated Chinese Hamster Ovary Cells (∗)
A Flynn, CG Proud - Journal of Biological Chemistry, 1995 - ASBMB
Ser-53 has previously been considered the major phosphorylation site in eukaryotic
initiation factor (eIF)-4E, and this appeared to be supported by studies using a S53A mutant.
Recently, however, several lines of evidence have indicated that Ser-53 might not be the
true phosphorylation site. This prompted us to re-examine the phosphorylation site in eIF-4E
using factor purified from 32 P-labeled, serum-treated Chinese hamster ovary cells.
Isoelectric focusing and phosphoamino acid analysis indicated the existence of a single …
initiation factor (eIF)-4E, and this appeared to be supported by studies using a S53A mutant.
Recently, however, several lines of evidence have indicated that Ser-53 might not be the
true phosphorylation site. This prompted us to re-examine the phosphorylation site in eIF-4E
using factor purified from 32 P-labeled, serum-treated Chinese hamster ovary cells.
Isoelectric focusing and phosphoamino acid analysis indicated the existence of a single …