[HTML][HTML] In vitro assembly and recognition of Lys-63 polyubiquitin chains
RM Hofmann, CM Pickart - Journal of Biological Chemistry, 2001 - ASBMB
Polyubiquitin chains assembled through lysine 48 (Lys-48) of ubiquitin act as a signal for
substrate proteolysis by 26 S proteasomes, whereas chains assembled through Lys-63 play
a mechanistically undefined role in post-replicative DNA repair. We showed previously that
the products of the UBC13 andMMS2 genes function in error-free post-replicative DNA
repair in the yeast Saccharomyces cerevisiae and form a complex that assembles Lys-63-
linked polyubiquitin chains in vitro. Here we confirm that the Mms2· Ubc13 complex …
substrate proteolysis by 26 S proteasomes, whereas chains assembled through Lys-63 play
a mechanistically undefined role in post-replicative DNA repair. We showed previously that
the products of the UBC13 andMMS2 genes function in error-free post-replicative DNA
repair in the yeast Saccharomyces cerevisiae and form a complex that assembles Lys-63-
linked polyubiquitin chains in vitro. Here we confirm that the Mms2· Ubc13 complex …