A simplified mycobacterial growth inhibition assay (MGIA) using direct infection of mouse splenocytes and the MGIT system
AL Yang, TE Schmidt, S Stibitz, SC Derrick… - Journal of …, 2016 - Elsevier
We describe a simplified Mycobacterial Growth Inhibition Assay (MGIA) for pre-clinical
assessment of vaccine-mediated protection in mice. The assay is accomplished by directly
infecting splenocytes from vaccinated mice with Mycobacterium tuberculosis and quantifying
mycobacteria using Mycobacterial Growth Indicator Tubes (MGIT). Vaccine-mediated
immunogenicity detected by this assay correlated with protection.
assessment of vaccine-mediated protection in mice. The assay is accomplished by directly
infecting splenocytes from vaccinated mice with Mycobacterium tuberculosis and quantifying
mycobacteria using Mycobacterial Growth Indicator Tubes (MGIT). Vaccine-mediated
immunogenicity detected by this assay correlated with protection.