Specificity and partial purification of a factor in spent media from Sertoli cell-enriched cultures that stimulates steroidogenesis in Leydig cells
G Verhoeven, J Cailleau - Journal of steroid biochemistry, 1986 - Elsevier
There is increasing evidence that factors derived from the seminiferous tubules influence
Leydig cell function in a paracrine way. In previous experiments we demonstrated that
conditioned media from Sertoli cell-enriched cultures contain a protein with stimulatory
activity on prepubertal rat Leydig cells. In this paper we further studied the specificity of this
factor. In addition we describe a simple but efficient partial purification procedure. It is
demonstrated that Sertoli cell conditioned media contain a factor that stimulates the …
Leydig cell function in a paracrine way. In previous experiments we demonstrated that
conditioned media from Sertoli cell-enriched cultures contain a protein with stimulatory
activity on prepubertal rat Leydig cells. In this paper we further studied the specificity of this
factor. In addition we describe a simple but efficient partial purification procedure. It is
demonstrated that Sertoli cell conditioned media contain a factor that stimulates the …