[HTML][HTML] CD44-dependent intracellular and extracellular catabolism of hyaluronic acid by hyaluronidase-1 and-2
H Harada, M Takahashi - Journal of Biological Chemistry, 2007 - ASBMB
Hyaluronic acid (HA) is a high molecular weight glycosaminoglycan involved in a wide
variety of cellular functions. However, its turnover in living cells remains largely unknown. In
this study, CD44, a receptor for HA, and hyaluronidase-1,-2, and-3 (Hyal-1,-2 and-3) were
stably expressed in HEK 293 cells and the mechanism of HA catabolism was systematically
investigated using fluorescein-labeled HA. CD44 was essential for HA degradation by both
endogenous and exogenously expressed hyaluronidases. Hyal-1 was not able to cleave HA …
variety of cellular functions. However, its turnover in living cells remains largely unknown. In
this study, CD44, a receptor for HA, and hyaluronidase-1,-2, and-3 (Hyal-1,-2 and-3) were
stably expressed in HEK 293 cells and the mechanism of HA catabolism was systematically
investigated using fluorescein-labeled HA. CD44 was essential for HA degradation by both
endogenous and exogenously expressed hyaluronidases. Hyal-1 was not able to cleave HA …