Highly multiplexed, single-cell technologies reveal important heterogeneity within cell
populations. Recently, technologies to simultaneously measure expression of 96 (or more)
genes from a single cell have been developed for immunologic monitoring. Here, we report
a rigorous, optimized, quantitative methodology for using this technology. Specifically: we
describe a unique primer/probe qualification method necessary for quantitative results; we
show that primers do not compete in highly multiplexed amplifications; we define the limit of …