Population genetic studies in the Congo. I. Glucose-6-phosphate dehydrogenase deficiency, hemoglobin S, and malaria.
AG Motulsky, J Vandepitte… - American Journal of …, 1966 - ncbi.nlm.nih.gov
AG Motulsky, J Vandepitte, GR Fraser
American Journal of Human Genetics, 1966•ncbi.nlm.nih.govMETHODS Laboratory Procedures A total of 786 blood specimens (Table 1A) were collected
by venepuncture into ACD solution and sent by air to Seattle in refrigerated containers. They
arrived in excellent condition for all relevant investigations. In addition to this sample which
was derived from eight populations, the frequencies of sickling (Hb S) and G6PD deficiency
were studied in four other groups in the Congo itself (Table 1B). Among the Yaka, six
villages within 50 km of Popokabaka (Kwango province of southwestern Congo) were …
by venepuncture into ACD solution and sent by air to Seattle in refrigerated containers. They
arrived in excellent condition for all relevant investigations. In addition to this sample which
was derived from eight populations, the frequencies of sickling (Hb S) and G6PD deficiency
were studied in four other groups in the Congo itself (Table 1B). Among the Yaka, six
villages within 50 km of Popokabaka (Kwango province of southwestern Congo) were …
METHODS
Laboratory Procedures A total of 786 blood specimens (Table 1A) were collected by venepuncture into ACD solution and sent by air to Seattle in refrigerated containers. They arrived in excellent condition for all relevant investigations. In addition to this sample which was derived from eight populations, the frequencies of sickling (Hb S) and G6PD deficiency were studied in four other groups in the Congo itself (Table 1B). Among the Yaka, six villages within 50 km of Popokabaka (Kwango province of southwestern Congo) were chosen in which the prevalence of malaria was still high (Ya 1-6). Blood from a total of 587 male children selected at random was taken by finger-prick each morning, kept in refrigerated containers, and tested in the late afternoon in an improvised field laboratory in Popokabaka for sickling and G6PD deficiency. Positive slide sickling tests were confirmed by paper electrophoresis using capillary blood. A count of malarial parasites (in this area almost exclusively Plasmodium falci-parum) was made for each specimen. The number of parasites in the" positive" thick drops was computed by enumerating in a large number of immer-
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