Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion
H Hu, M Li, JP Labrador, J McEwen… - Proceedings of the …, 2005 - National Acad Sciences
Proceedings of the National Academy of Sciences, 2005•National Acad Sciences
The regulators of the Rho-family GTPases, GTPase-activating proteins (GAPs) and guanine
exchange factors (GEFs), play important roles in axon guidance. By means of a functional
genomic study of the Rho-family GEFs and GAPs in Drosophila, we have identified a Rho-
family GAP, CrossGAP (CrGAP), which is involved in Roundabout (Robo) receptor-mediated
repulsive axon guidance. CrGAP physically associates with the Robo receptor. Too much or
too little CrGAP activity leads to defects in Robo-mediated repulsion at the midline choice …
exchange factors (GEFs), play important roles in axon guidance. By means of a functional
genomic study of the Rho-family GEFs and GAPs in Drosophila, we have identified a Rho-
family GAP, CrossGAP (CrGAP), which is involved in Roundabout (Robo) receptor-mediated
repulsive axon guidance. CrGAP physically associates with the Robo receptor. Too much or
too little CrGAP activity leads to defects in Robo-mediated repulsion at the midline choice …
The regulators of the Rho-family GTPases, GTPase-activating proteins (GAPs) and guanine exchange factors (GEFs), play important roles in axon guidance. By means of a functional genomic study of the Rho-family GEFs and GAPs in Drosophila, we have identified a Rho-family GAP, CrossGAP (CrGAP), which is involved in Roundabout (Robo) receptor-mediated repulsive axon guidance. CrGAP physically associates with the Robo receptor. Too much or too little CrGAP activity leads to defects in Robo-mediated repulsion at the midline choice point. The CrGAP gain-of-function phenotype mimics the loss-of-function phenotypes of both Robo and Rac. Dosage-sensitive genetic interactions among CrGAP, Robo, and Rac support a model in which CrGAP transduces signals downstream of Robo receptor to regulate Rac-dependent cytoskeletal changes.
National Acad Sciences