Rescue of recombinant Newcastle disease virus from cDNA
J Ayllon, A García-Sastre… - JoVE (Journal of Visualized …, 2013 - jove.com
Newcastle disease virus (NDV), the prototype member of the Avulavirus genus of the family
Paramyxoviridae1, is a non-segmented, negative-sense, single-stranded, enveloped RNA
virus (Figure 1) with potential applications as a vector for vaccination and treatment of
human diseases. In-depth exploration of these applications has only become possible after
the establishment of reverse genetics techniques to rescue recombinant viruses from
plasmids encoding their complete genomes as cDNA2-5. Viral cDNA can be conveniently …
Paramyxoviridae1, is a non-segmented, negative-sense, single-stranded, enveloped RNA
virus (Figure 1) with potential applications as a vector for vaccination and treatment of
human diseases. In-depth exploration of these applications has only become possible after
the establishment of reverse genetics techniques to rescue recombinant viruses from
plasmids encoding their complete genomes as cDNA2-5. Viral cDNA can be conveniently …