Therapeutic effects of a fusogenic newcastle disease virus in treating head and neck cancer
Head & neck, 2011•Wiley Online Library
Background Newcastle disease virus (NDV) is a paramyxovirus that is pathogenic in birds
but causes only mild flulike symptoms in human beings. NDV (F3aa)‐GFP is a genetically
modified, fusogenic NDV. We assessed the utility of NDV (F3aa)‐GFP in treating head and
neck squamous cell carcinoma. Methods and Results At a multiplicity of infection (MOI) of 1,
NDV (F3aa)‐GFP infection of 3 cell lines supported strong GFP expression by 36 hours.
Four cell lines were highly sensitivite to viral cytotoxicity, with> 75% of cells lysed by day 6 at …
but causes only mild flulike symptoms in human beings. NDV (F3aa)‐GFP is a genetically
modified, fusogenic NDV. We assessed the utility of NDV (F3aa)‐GFP in treating head and
neck squamous cell carcinoma. Methods and Results At a multiplicity of infection (MOI) of 1,
NDV (F3aa)‐GFP infection of 3 cell lines supported strong GFP expression by 36 hours.
Four cell lines were highly sensitivite to viral cytotoxicity, with> 75% of cells lysed by day 6 at …
Background
Newcastle disease virus (NDV) is a paramyxovirus that is pathogenic in birds but causes only mild flulike symptoms in human beings. NDV(F3aa)‐GFP is a genetically modified, fusogenic NDV. We assessed the utility of NDV(F3aa)‐GFP in treating head and neck squamous cell carcinoma.
Methods and Results
At a multiplicity of infection (MOI) of 1, NDV(F3aa)‐GFP infection of 3 cell lines supported strong GFP expression by 36 hours. Four cell lines were highly sensitivite to viral cytotoxicity, with >75% of cells lysed by day 6 at MOI 0.1, and 2 other cell lines were partially susceptible. Murine SCC25 flank tumors exhibited robust GFP expression after a single intratumoral viral injection and showed near‐complete tumor regression over 34 days. There were no adverse effects attributable to therapy.
Conclusions
We demonstrate that a fusogenic NDV exerts potent oncolytic effects against human head and neck cancer and support its continued investigation for clinical application. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
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