[HTML][HTML] Eosinophil peroxidase oxidation of thiocyanate: characterization of major reaction products and a potential sulfhydryl-targeted cytotoxicity system
M Arlandson, T Decker, VA Roongta, L Bonilla… - Journal of Biological …, 2001 - ASBMB
Although the pseudohalide thiocyanate (SCN−) is the preferred substrate for eosinophil
peroxidase (EPO) in fluids of physiologic halide composition, the product (s) of this reaction
have not been directly identified, and mechanisms underlying their cytotoxic potential are
poorly characterized. We used nuclear magnetic resonance spectroscopy (NMR),
electrospray ionization mass spectrometry, and quantitative chemical analysis to identify the
principal reaction products of both the EPO/SCN−/H 2 O 2 system and activated eosinophils …
peroxidase (EPO) in fluids of physiologic halide composition, the product (s) of this reaction
have not been directly identified, and mechanisms underlying their cytotoxic potential are
poorly characterized. We used nuclear magnetic resonance spectroscopy (NMR),
electrospray ionization mass spectrometry, and quantitative chemical analysis to identify the
principal reaction products of both the EPO/SCN−/H 2 O 2 system and activated eosinophils …