[HTML][HTML] Preanalytic variables of thrombin generation: towards a standard procedure and validation of the method

R Loeffen, MCF Kleinegris, S Loubele… - Journal of thrombosis …, 2012 - Elsevier
Background: Thrombin generation assays are sensitive methods for assessment of the
overall clotting potential of plasma, but, despite their common use in thrombosis research,
standardization of preanalytic conditions is lacking. In order to set up a standardized
protocol, we analyzed different preanalytic variables and validated the calibrated automated
thrombogram method. Methods and Results: Thrombin generation was assessed with 0, 1
and 5 pm tissue factor (TF). Variations in thrombin generation were mostly attributable to the …