Iron-induced oxidative brain injury after experimental intracerebral hemorrhage
T Nakamura, RF Keep, Y Hua, S Nagao, JT Hoff, G Xi - Brain Edema XIII, 2006 - Springer
T Nakamura, RF Keep, Y Hua, S Nagao, JT Hoff, G Xi
Brain Edema XIII, 2006•SpringerWe investigated the occurrence of DNA damage in brain after intracerebral hemorrhage
(ICH) and the role of iron in such injury. Male Sprague-Dawley rats received an infusion of
100 µL autologous whole blood or 30 µL FeCl 2 into the right basal ganglia and were
sacrificed 1, 3, or 7 days later. 8-hydroxyl-2′-deoxyguanosine (8-OHdG) was analyzed by
immunohistochemistry, while the number of apurinic/apyrimidinic abasic sites (AP sites) was
also quantified. 8-OHdG and AP sites are two hallmarks of DNA oxidation. DNA damage …
(ICH) and the role of iron in such injury. Male Sprague-Dawley rats received an infusion of
100 µL autologous whole blood or 30 µL FeCl 2 into the right basal ganglia and were
sacrificed 1, 3, or 7 days later. 8-hydroxyl-2′-deoxyguanosine (8-OHdG) was analyzed by
immunohistochemistry, while the number of apurinic/apyrimidinic abasic sites (AP sites) was
also quantified. 8-OHdG and AP sites are two hallmarks of DNA oxidation. DNA damage …
Summary
We investigated the occurrence of DNA damage in brain after intracerebral hemorrhage (ICH) and the role of iron in such injury.
Male Sprague-Dawley rats received an infusion of 100 µL autologous whole blood or 30 µL FeCl2 into the right basal ganglia and were sacrificed 1, 3, or 7 days later. 8-hydroxyl-2′-deoxyguanosine (8-OHdG) was analyzed by immunohistochemistry, while the number of apurinic/apyrimidinic abasic sites (AP sites) was also quantified. 8-OHdG and AP sites are two hallmarks of DNA oxidation. DNA damage was also examined using PANT and TUNEL labeling. Dinitrophenyl (DNP) was measured by Western blot to compare the time course of protein oxidative damage to that of DNA. DNA repair APE/Ref-1 and Ku-proteins were also measured by Western blot. Bipyridine, a ferrous iron chelator, was used to examine the role of iron in ICH-induced oxidative brain injury.
An increase in 8-OHdG, AP sites, and DNP levels, and a decrease in APE/Ref-1 and Ku levels were observed. Abundant PANT-positive cells were also observed in the perihematomal area 3 days after ICH. Bipyridine attenuated ICH-induced changes in PANT and DNP. These results suggest that iron-induced oxidation causes DNA damage in brain after ICH and that iron is a therapeutic target for ICH.
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