Floxin, a resource for genetically engineering mouse ESCs
V Singla, J Hunkapiller, N Santos, AD Seol… - Nature …, 2010 - nature.com
V Singla, J Hunkapiller, N Santos, AD Seol, AR Norman, P Wakenight, WC Skarnes…
Nature methods, 2010•nature.comWe describe a method for the highly efficient and precise targeted modification of gene trap
loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations
were reverted and new DNA sequences inserted using Cre recombinase and a shuttle
vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149
compatible gene trap cell lines, which should enable high-throughput modification of many
genes in mouse ESCs.
loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations
were reverted and new DNA sequences inserted using Cre recombinase and a shuttle
vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149
compatible gene trap cell lines, which should enable high-throughput modification of many
genes in mouse ESCs.
Abstract
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.
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