Activation signal of nuclear factor-κB in response to endoplasmic reticulum stress is transduced via IRE1 and tumor necrosis factor receptor-associated factor 2
M Kaneko, Y Niinuma, Y Nomura - Biological and Pharmaceutical …, 2003 - jstage.jst.go.jp
M Kaneko, Y Niinuma, Y Nomura
Biological and Pharmaceutical Bulletin, 2003•jstage.jst.go.jpIRE1a (the region corresponding to amino acids; 1—977) and DRN-IRE1a (a truncated form
of the C-terminal RNase domain; 1—786) with a hemagglutinin (HA) epitope at the C-
terminus were amplified by PCR. K599A-IRE1a mutant was constructed by PCR using the
overlapping method. All were ligated into the vector pCR3. 1 (Invitrogen). Human full-length
TRAF2 (1—508) and DN-TRAF2 (a N-terminustruncated form; 87—508) were amplified by
PCR, and ligated into the vector pcDNA6/Myc-His (Invitrogen), expressing Myc (c-Myc) and …
of the C-terminal RNase domain; 1—786) with a hemagglutinin (HA) epitope at the C-
terminus were amplified by PCR. K599A-IRE1a mutant was constructed by PCR using the
overlapping method. All were ligated into the vector pCR3. 1 (Invitrogen). Human full-length
TRAF2 (1—508) and DN-TRAF2 (a N-terminustruncated form; 87—508) were amplified by
PCR, and ligated into the vector pcDNA6/Myc-His (Invitrogen), expressing Myc (c-Myc) and …
IRE1a (the region corresponding to amino acids; 1—977) and DRN-IRE1a (a truncated form of the C-terminal RNase domain; 1—786) with a hemagglutinin (HA) epitope at the C-terminus were amplified by PCR. K599A-IRE1a mutant was constructed by PCR using the overlapping method. All were ligated into the vector pCR3. 1 (Invitrogen). Human full-length TRAF2 (1—508) and DN-TRAF2 (a N-terminustruncated form; 87—508) were amplified by PCR, and ligated into the vector pcDNA6/Myc-His (Invitrogen), expressing Myc (c-Myc) and polyhistidine (6His) epitopes at the C-terminus of the inserted sequence.
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