Molecular basis of the biological function of molybdenum: molybdenum-free sulfite oxidase from livers of tungsten-treated rats
JL Johnson, HJ Cohen, KV Rajagopalan - Journal of Biological Chemistry, 1974 - ASBMB
Livers of tungsten-treated rats have been shown to contain inactive protein which cross-
reacts with antibody prepared against native rat liver sulfite oxidase. The cross-reacting
material (CRM) was shown to be immunologically identical to the native enzyme by
Ouchterlony double immunodiffusion. Difference spectrophotometry, acrylamide gel
electrophoresis, and immunological analysis of the proteins of the mitochondrial
intermembranous space from livers of control and tungsten-treated rats showed that, like …
reacts with antibody prepared against native rat liver sulfite oxidase. The cross-reacting
material (CRM) was shown to be immunologically identical to the native enzyme by
Ouchterlony double immunodiffusion. Difference spectrophotometry, acrylamide gel
electrophoresis, and immunological analysis of the proteins of the mitochondrial
intermembranous space from livers of control and tungsten-treated rats showed that, like …