p38 mitogen-activated protein kinase activation during platelet storage: consequences for platelet recovery and hemostatic function in vivo

M Canault, D Duerschmied, A Brill… - Blood, The Journal …, 2010 - ashpublications.org
M Canault, D Duerschmied, A Brill, L Stefanini, D Schatzberg, SM Cifuni, W Bergmeier
Blood, The Journal of the American Society of Hematology, 2010ashpublications.org
Platelets undergo several modifications during storage that reduce their posttransfusion
survival and functionality. One important feature of these changes, which are known as
platelet storage lesion, is the shedding of the surface glycoproteins GPIb-α and GPV. We
recently demonstrated that tumor necrosis factor-α converting enzyme (TACE/ADAM17)
mediates mitochondrial injury-induced shedding of adhesion receptors and that TACE
activity correlates with reduced posttransfusion survival of these cells. We now confirm that …
Abstract
Platelets undergo several modifications during storage that reduce their posttransfusion survival and functionality. One important feature of these changes, which are known as platelet storage lesion, is the shedding of the surface glycoproteins GPIb-α and GPV. We recently demonstrated that tumor necrosis factor-α converting enzyme (TACE/ADAM17) mediates mitochondrial injury-induced shedding of adhesion receptors and that TACE activity correlates with reduced posttransfusion survival of these cells. We now confirm that TACE mediates receptor shedding and clearance of platelets stored for 16 hours at 37°C or 22°C. We further demonstrate that both storage and mitochondrial injury lead to the phosphorylation of p38 mitogen-activated kinase (MAPK) in platelets and that TACE-mediated receptor shedding from mouse and human platelets requires p38 MAP kinase signaling. Protein kinase C, extracellular regulated-signal kinase MAPK, and caspases were not involved in TACE activation. Both inhibition of p38 MAPK and inactivation of TACE during platelet storage led to a markedly improved posttransfusion recovery and hemostatic function of platelets in mice. p38 MAPK inhibitors had only minor effects on the aggregation of fresh platelets under static or flow conditions in vitro. In summary, our data suggest that inhibition of p38 MAPK or TACE during storage may significantly improve the quality of stored platelets.
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