[HTML][HTML] Association of villin with phosphatidylinositol 4, 5-bisphosphate regulates the actin cytoskeleton
Villin, an epithelial cell actin-binding protein, severs actin in vitro and in vivo. Previous
studies report that phosphatidylinositol 4, 5-bisphosphate (PIP 2) regulates actin severing by
villin, presumably by interaction with villin. However, direct association of villin with PIP 2
has never been characterized. In this report, we presented mutational analysis to identify the
PIP 2-binding sites in villin. Villin (human) binds PIP 2 with a K d of 39.5 μm, a stoichiometry
of 3.3, and a Hill coefficient of 1. We generated deletion mutants of villin lacking putative PIP …
studies report that phosphatidylinositol 4, 5-bisphosphate (PIP 2) regulates actin severing by
villin, presumably by interaction with villin. However, direct association of villin with PIP 2
has never been characterized. In this report, we presented mutational analysis to identify the
PIP 2-binding sites in villin. Villin (human) binds PIP 2 with a K d of 39.5 μm, a stoichiometry
of 3.3, and a Hill coefficient of 1. We generated deletion mutants of villin lacking putative PIP …