Publisher Summary Recombinant adeno-associated virus (rAAV), because of its simplicity,
ability to infect a wide variety of dividing and nondividing cells, and lack of human
pathogenicity, has proved to be a useful vector for efficient and long-term gene transfer in
vivo. The traditional rAAV purification protocol involved the stepwise precipitation of rAAV
with ammonium sulfate, followed by two or three rounds of CsC1 density gradient
centrifugation. Each gradient required fractionation and identification of the virus-containing …