Heat induces γH2AX foci formation in mammalian cells
A Takahashi, E Mori, GI Somakos, K Ohnishi… - … /Genetic Toxicology and …, 2008 - Elsevier
H2AX is a histone variant which is present and ubiquitously distributed throughout the
genome. An immunocytochemical assay using antibodies capable of recognizing histone
H2AX phosphorylated at serine 139 (γH2AX) is very sensitive and is a specific indicator for
the existence of a DNA double strand break. Although heat stress has been reported to
induce the formation of γH2AX foci, no γH2AX foci formation was observed in several
mammalian cell lines after heat shock. Since this was in contrast to earlier reports, the work …
genome. An immunocytochemical assay using antibodies capable of recognizing histone
H2AX phosphorylated at serine 139 (γH2AX) is very sensitive and is a specific indicator for
the existence of a DNA double strand break. Although heat stress has been reported to
induce the formation of γH2AX foci, no γH2AX foci formation was observed in several
mammalian cell lines after heat shock. Since this was in contrast to earlier reports, the work …