Recombinant human factor X: high yield expression and the role of furin in proteolytic maturation in vivo and in vitro
M Himmelspach, M Pfleiderer, BE Fischer… - Thrombosis research, 2000 - Elsevier
Factor X/Xa plays a pivotal role in the coagulation cascade and exhibits a therapeutic
potential for the treatment of factor X–deficient as well as FVIII and FIX inhibitor patients. This
report describes the establishment of Chinese hamster ovary cell clones expressing
recombinant human factor X up to 120 μg/mL× day and 78 μg/106cells× day, that is to 100-
fold higher levels than reported previously. Although propeptide removal and single chain
precursor to light and heavy chain processing as well as vitamin K–dependent γ …
potential for the treatment of factor X–deficient as well as FVIII and FIX inhibitor patients. This
report describes the establishment of Chinese hamster ovary cell clones expressing
recombinant human factor X up to 120 μg/mL× day and 78 μg/106cells× day, that is to 100-
fold higher levels than reported previously. Although propeptide removal and single chain
precursor to light and heavy chain processing as well as vitamin K–dependent γ …