[HTML][HTML] The molecular basis for ligand specificity in a mouse olfactory receptor: a network of functionally important residues
T Abaffy, A Malhotra, CW Luetje - Journal of biological chemistry, 2007 - ASBMB
Sequence differences between members of the mouse olfac-tory receptor MOR42 subfamily
(MOR42-3 and MOR42-1) are likely to be the basis for variation in ligand binding preference
among these receptors. We investigated the specificity of MOR42-3 for a variety of
dicarboxylic acids. We used site-directed mutagenesis, guided by homology modeling and
ligand docking studies, to locate functionally important residues. Receptors were expressed
in Xenopus oocytes and assayed using high throughput electrophysiology. The importance …
(MOR42-3 and MOR42-1) are likely to be the basis for variation in ligand binding preference
among these receptors. We investigated the specificity of MOR42-3 for a variety of
dicarboxylic acids. We used site-directed mutagenesis, guided by homology modeling and
ligand docking studies, to locate functionally important residues. Receptors were expressed
in Xenopus oocytes and assayed using high throughput electrophysiology. The importance …