SRP19 Is a Dispensable Component of the Signal Recognition Particle in Archaea
S Yurist, I Dahan, J Eichler - Journal of bacteriology, 2007 - Am Soc Microbiol
S Yurist, I Dahan, J Eichler
Journal of bacteriology, 2007•Am Soc MicrobiolIn vitro, archaeal SRP54 binds SRP RNA in the absence of SRP19, suggesting the latter to
be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted.
Although normally transcribed at a level comparable to that of the essential SRP54 gene,
SRP19 deletion had no effect on cell growth, membrane protein insertion, protein secretion,
or ribosome levels. The absence of SRP19 did, however, increase membrane
bacterioruberin levels.
be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted.
Although normally transcribed at a level comparable to that of the essential SRP54 gene,
SRP19 deletion had no effect on cell growth, membrane protein insertion, protein secretion,
or ribosome levels. The absence of SRP19 did, however, increase membrane
bacterioruberin levels.
Abstract
In vitro, archaeal SRP54 binds SRP RNA in the absence of SRP19, suggesting the latter to be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted. Although normally transcribed at a level comparable to that of the essential SRP54 gene, SRP19 deletion had no effect on cell growth, membrane protein insertion, protein secretion, or ribosome levels. The absence of SRP19 did, however, increase membrane bacterioruberin levels.
American Society for Microbiology