L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein
C Kocks, E Gouin, M Tabouret, P Berche, H Ohayon… - Cell, 1992 - cell.com
C Kocks, E Gouin, M Tabouret, P Berche, H Ohayon, P Cossart
Cell, 1992•cell.comThe intracellular pathogenic bacterium L. monocytogenes can spread directly from cell to
cell without leaving the cytoplasm. The mechanism of this movement, generated through
bacterially induced actin polymerization, is not understood. By analyzing an avirulent
Tn9174ac mutant defective for actin polymerization, we have identified a bacterial
component involved in this process. The transposon had inserted in actA, the second gene
of an operon. Gene disruption of downstream genes and transformation of the mutant strain …
cell without leaving the cytoplasm. The mechanism of this movement, generated through
bacterially induced actin polymerization, is not understood. By analyzing an avirulent
Tn9174ac mutant defective for actin polymerization, we have identified a bacterial
component involved in this process. The transposon had inserted in actA, the second gene
of an operon. Gene disruption of downstream genes and transformation of the mutant strain …
Summary
The intracellular pathogenic bacterium L. monocytogenes can spread directly from cell to cell without leaving the cytoplasm. The mechanism of this movement, generated through bacterially induced actin polymerization, is not understood. By analyzing an avirulent Tn9174ac mutant defective for actin polymerization, we have identified a bacterial component involved in this process. The transposon had inserted in actA, the second gene of an operon. Gene disruption of downstream genes and transformation of the mutant strain with actA showed that the actA gene encodes a surface protein necessary for bacterially induced actin assembly. Our results indicate that it is a 610 amino acid protein with an apparent molecular weight of 90 kd.
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