[HTML][HTML] Epigenetic activation of a subset of mRNAs by eIF4E explains its effects on cell proliferation

Y Mamane, E Petroulakis, Y Martineau, TA Sato… - PloS one, 2007 - journals.plos.org
Y Mamane, E Petroulakis, Y Martineau, TA Sato, O Larsson, VK Rajasekhar, N Sonenberg
PloS one, 2007journals.plos.org
Background Translation deregulation is an important mechanism that causes aberrant cell
growth, proliferation and survival. eIF4E, the mRNA 5′ cap-binding protein, plays a major
role in translational control. To understand how eIF4E affects cell proliferation and survival,
we studied mRNA targets that are translationally responsive to eIF4E. Methodology/Principal
Findings Microarray analysis of polysomal mRNA from an eIF4E-inducible NIH 3T3 cell line
was performed. Inducible expression of eIF4E resulted in increased translation of defined …
Background
Translation deregulation is an important mechanism that causes aberrant cell growth, proliferation and survival. eIF4E, the mRNA 5′ cap-binding protein, plays a major role in translational control. To understand how eIF4E affects cell proliferation and survival, we studied mRNA targets that are translationally responsive to eIF4E.
Methodology/Principal Findings
Microarray analysis of polysomal mRNA from an eIF4E-inducible NIH 3T3 cell line was performed. Inducible expression of eIF4E resulted in increased translation of defined sets of mRNAs. Many of the mRNAs are novel targets, including those that encode large- and small-subunit ribosomal proteins and cell growth-related factors. In addition, there was augmented translation of mRNAs encoding anti-apoptotic proteins, which conferred resistance to endoplasmic reticulum-mediated apoptosis.
Conclusions/Significance
Our results shed new light on the mechanisms by which eIF4E prevents apoptosis and transforms cells. Downregulation of eIF4E and its downstream targets is a potential therapeutic option for the development of novel anti-cancer drugs.
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