Screening of fusion partners for high yield expression and purification of bioactive viscotoxins

J Bogomolovas, B Simon, M Sattler, G Stier - Protein expression and …, 2009 - Elsevier
Viscotoxins are small cationic proteins found in European mistletoe Viscum album. They are
highly toxic towards phytopathogenic fungi and cancer cells. Heterologous expression of
viscotoxins would broaden the spectrum of methods to be applied for better understanding
of their structure and function and satisfy possible biopharmaceutical needs. Here, we
evaluated 13 different proteins as a fusion partners for expression in Escherichia coli cells:
His6 tag and His6-tagged versions of GB1, ZZ tag, Z tag, maltose binding protein, NusA …