[HTML][HTML] Accurate quantification of more than 4000 mouse tissue proteins reveals minimal proteome changes during aging
DM Walther, M Mann - Molecular & Cellular Proteomics, 2011 - ASBMB
The biological process of aging is believed to be the result of an accumulation of cellular
damage to biomolecules. Although there are numerous studies addressing mutation
frequencies, morphological or transcriptional changes in aging mammalian tissues, few
have measured global changes at the protein level. Here, we present an in depth proteomic
analysis of three brain regions as well as heart and kidney in mice aged 5 or 26 months,
using stable isotope labeling of whole animals (SILAC mouse) and high resolution mass …
damage to biomolecules. Although there are numerous studies addressing mutation
frequencies, morphological or transcriptional changes in aging mammalian tissues, few
have measured global changes at the protein level. Here, we present an in depth proteomic
analysis of three brain regions as well as heart and kidney in mice aged 5 or 26 months,
using stable isotope labeling of whole animals (SILAC mouse) and high resolution mass …