TaqMan RT-PCR and VERSANT® HIV-1 RNA 3.0 (bDNA) assay: Quantification of HIV-1 RNA viral load in breast milk
K Israel-Ballard, R Ziermann, C Leutenegger… - Journal of clinical …, 2005 - Elsevier
Background: Transmission of HIV via breast milk is a primary cause of pediatric HIV infection
in developing countries. Reliable methods to detect breast milk viral load are important.
Objective: To correlate the ability of the VERSANT HIV 3.0 (bDNA) assay to real-time (RT)
TaqMan PCR in quantifying breast milk HIV-1 RNA. Study design: Forty-six breast milk
samples that had been spiked with cell-free HIV-1 and eight samples spiked with cell-
associated HIV-1 were assayed for HIV-1 RNA by both VERSANT HIV 3.0 and TaqMan RNA …
in developing countries. Reliable methods to detect breast milk viral load are important.
Objective: To correlate the ability of the VERSANT HIV 3.0 (bDNA) assay to real-time (RT)
TaqMan PCR in quantifying breast milk HIV-1 RNA. Study design: Forty-six breast milk
samples that had been spiked with cell-free HIV-1 and eight samples spiked with cell-
associated HIV-1 were assayed for HIV-1 RNA by both VERSANT HIV 3.0 and TaqMan RNA …