[HTML][HTML] E3 ligase RFWD3 participates in replication checkpoint control
Z Gong, J Chen - Journal of Biological Chemistry, 2011 - ASBMB
RFWD3 has E3 ligase activity in vitro, but its in vivo function remains unknown. In this study
we identified RFWD3 as a novel replication protein A (RPA)-associated protein. Using
purified proteins, we observed a direct interaction between RPA2 and RFWD3. Further
analysis showed that RFWD3 is recruited to stalled replication forks and co-localizes with
RPA2 in response to replication stress. Moreover, RFWD3 is important for ATR-dependent
Chk1 activation in response to replication stress. Upon replication stress, deletion of RPA2 …
we identified RFWD3 as a novel replication protein A (RPA)-associated protein. Using
purified proteins, we observed a direct interaction between RPA2 and RFWD3. Further
analysis showed that RFWD3 is recruited to stalled replication forks and co-localizes with
RPA2 in response to replication stress. Moreover, RFWD3 is important for ATR-dependent
Chk1 activation in response to replication stress. Upon replication stress, deletion of RPA2 …