Detection and isolation of cytokine secreting cells using the cytometric cytokine secretion assay
M Assenmacher, M Löhning… - Current protocols in …, 2001 - Wiley Online Library
M Assenmacher, M Löhning, A Radbruch
Current protocols in immunology, 2001•Wiley Online LibraryThe cell‐surface affinity matrix technology, otherwise called “secretion assay” or “capture
assay,” represents a new, innovative method for the analysis and enrichment of viable cells
according to secreted molecules such as antibodies or cytokineof T cells to induce cytokine
secretion is included. Alternate protocols provide very rapid and simple procedures for the
direct detection of antigen‐specific T cells from small numbers of PBMCs or whole blood
without magnetic enrichment.
assay,” represents a new, innovative method for the analysis and enrichment of viable cells
according to secreted molecules such as antibodies or cytokineof T cells to induce cytokine
secretion is included. Alternate protocols provide very rapid and simple procedures for the
direct detection of antigen‐specific T cells from small numbers of PBMCs or whole blood
without magnetic enrichment.
Abstract
The cell‐surface affinity matrix technology, otherwise called “secretion assay” or “capture assay,” represents a new, innovative method for the analysis and enrichment of viable cells according to secreted molecules such as antibodies or cytokineof T cells to induce cytokine secretion is included. Alternate protocols provide very rapid and simple procedures for the direct detection of antigen‐specific T cells from small numbers of PBMCs or whole blood without magnetic enrichment.
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