Determination of lymphocyte division by flow cytometry

AB Lyons, CR Parish - Journal of immunological methods, 1994 - Elsevier
Techniques currently available for determining cell division are able to show one or, at best,
a limited number of cell divisions. Other methods exist which can quantify overall division,
but tell nothing about the division history of individual cells. Here we present a new
technique is which an intracellular fluorescent label is divided equally between daughter
cells upon cell division. The technique is applicable to in vitro cell division, as well as in vivo
division of adoptively transferred cells, and can resolve multiple successive generations …