[HTML][HTML] TCR repertoire analysis by next generation sequencing allows complex differential diagnosis of T cell–related pathology
M Dziubianau, J Hecht, L Kuchenbecker… - American journal of …, 2013 - Elsevier
Clonotype analysis is essential for complete characterization of antigen-specific T cells.
Moreover, knowledge on clonal identity allows tracking of antigen-specific T cells in whole
blood and tissue infiltrates and can provide information on antigenic specificity. Here, we
developed a next generation sequencing (NGS)-based platform for the highly quantitative
clonotype characterization of T cells and determined requirements for the unbiased
characterization of the input material (DNA, RNA, ex vivo derived or cell culture expanded T …
Moreover, knowledge on clonal identity allows tracking of antigen-specific T cells in whole
blood and tissue infiltrates and can provide information on antigenic specificity. Here, we
developed a next generation sequencing (NGS)-based platform for the highly quantitative
clonotype characterization of T cells and determined requirements for the unbiased
characterization of the input material (DNA, RNA, ex vivo derived or cell culture expanded T …