IFNγ production by NK cells from HLA-sensitized patients after in vitro exposure to allo-antigens
M Toyoda, S Ge, E Suviolahti, P Pichurin, B Shin… - Transplant …, 2012 - Elsevier
Using a novel cytokine flow cytometry test (allo-CFC), we have previously shown that
incubation of allogeneic cells with peripheral blood from highly-HLA sensitized (HS) patients
results in reproducible gamma-interferon (IFNγ production in CD3− cells, and high (+) allo-
CFC levels correlated with risk for antibody-mediated rejection (AMR). Here we report on
identification of the cells and mechanisms responsible. The allo-CFC with/without
modification was performed using blood from HS or normal individuals. IFNγ producing cells …
incubation of allogeneic cells with peripheral blood from highly-HLA sensitized (HS) patients
results in reproducible gamma-interferon (IFNγ production in CD3− cells, and high (+) allo-
CFC levels correlated with risk for antibody-mediated rejection (AMR). Here we report on
identification of the cells and mechanisms responsible. The allo-CFC with/without
modification was performed using blood from HS or normal individuals. IFNγ producing cells …