[PDF][PDF] Addiction of t (8; 21) and inv (16) acute myeloid leukemia to native RUNX1

O Ben-Ami, D Friedman, D Leshkowitz, D Goldenberg… - Cell reports, 2013 - cell.com
O Ben-Ami, D Friedman, D Leshkowitz, D Goldenberg, K Orlovsky, N Pencovich, J Lotem…
Cell reports, 2013cell.com
Summary The t (8; 21) and inv (16) chromosomal aberrations generate the oncoproteins
AML1-ETO (AE) and CBFβ-SMMHC (CS). The role of these oncoproteins in acute myeloid
leukemia (AML) etiology has been well studied. Conversely, the function of native RUNX1 in
promoting AE-and CS-mediated leukemias has remained elusive. We show that wild-type
RUNX1 is required for the survival of t (8; 21)-Kasumi-1 and inv (16)-ME-1 leukemic cells.
RUNX1 knockdown in Kasumi-1 cells (Kasumi-1 RX1-KD) attenuates the cell-cycle mitotic …
Summary
The t(8;21) and inv(16) chromosomal aberrations generate the oncoproteins AML1-ETO (A-E) and CBFβ-SMMHC (C-S). The role of these oncoproteins in acute myeloid leukemia (AML) etiology has been well studied. Conversely, the function of native RUNX1 in promoting A-E- and C-S-mediated leukemias has remained elusive. We show that wild-type RUNX1 is required for the survival of t(8;21)-Kasumi-1 and inv(16)-ME-1 leukemic cells. RUNX1 knockdown in Kasumi-1 cells (Kasumi-1RX1-KD) attenuates the cell-cycle mitotic checkpoint, leading to apoptosis, whereas knockdown of A-E in Kasumi-1RX1-KD rescues these cells. Mechanistically, a delicate RUNX1/A-E balance involving competition for common genomic sites that regulate RUNX1/A-E targets sustains the malignant cell phenotype. The broad medical significance of this leukemic cell addiction to native RUNX1 is underscored by clinical data showing that an active RUNX1 allele is usually preserved in both t(8;21) or inv(16) AML patients, whereas RUNX1 is frequently inactivated in other forms of leukemia. Thus, RUNX1 and its mitotic control targets are potential candidates for new therapeutic approaches.
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