[HTML][HTML] Quantitation of pyrrole-imidazole polyamide in rat plasma by high-performance liquid chromatography coupled with UV detection
T Kamei, T Aoyama, C Tanaka, T Nagashima… - BioMed Research …, 2012 - hindawi.com
A simple and robust method using high-performance liquid chromatography with UV
detection was developed and validated for the determination of six pyrrole-imidazole (PI)
polyamides (HN. 49, TGF-β1f, TGF-β1t, HN. 50f, HN. 50t, and LOX-1) in rat plasma. After the
plasma proteins were precipitated with methanol containing phenacetin as an internal
standard, the analytes were separated on a Luna C18 (2)(5 μm, 4. 6× 1 5 0 mm). Calibration
curves were linear over the range of 0.5 to 200 μg/mL for HN. 49, 0.25 to 200 μg/mL for TGF …
detection was developed and validated for the determination of six pyrrole-imidazole (PI)
polyamides (HN. 49, TGF-β1f, TGF-β1t, HN. 50f, HN. 50t, and LOX-1) in rat plasma. After the
plasma proteins were precipitated with methanol containing phenacetin as an internal
standard, the analytes were separated on a Luna C18 (2)(5 μm, 4. 6× 1 5 0 mm). Calibration
curves were linear over the range of 0.5 to 200 μg/mL for HN. 49, 0.25 to 200 μg/mL for TGF …
[PDF][PDF] Research Article Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection
T Kamei, T Aoyama, C Tanaka, T Nagashima… - 2012 - academia.edu
A simple and robust method using high-performance liquid chromatography with UV
detection was developed and validated for the determination of six pyrrole-imidazole (PI)
polyamides (HN. 49, TGF-β1f, TGF-β1t, HN. 50f, HN. 50t, and LOX-1) in rat plasma. After the
plasma proteins were precipitated with methanol containing phenacetin as an internal
standard, the analytes were separated on a Luna C18 (2)(5 µm, 4. 6× 150 mm). Calibration
curves were linear over the range of 0.5 to 200 µg/mL for HN. 49, 0.25 to 200 µg/mL for TGF …
detection was developed and validated for the determination of six pyrrole-imidazole (PI)
polyamides (HN. 49, TGF-β1f, TGF-β1t, HN. 50f, HN. 50t, and LOX-1) in rat plasma. After the
plasma proteins were precipitated with methanol containing phenacetin as an internal
standard, the analytes were separated on a Luna C18 (2)(5 µm, 4. 6× 150 mm). Calibration
curves were linear over the range of 0.5 to 200 µg/mL for HN. 49, 0.25 to 200 µg/mL for TGF …