[HTML][HTML] Requirement for endocytic antigen processing and influence of invariant chain and H-2M deficiencies in CNS autoimmunity

AJ Slavin, JM Soos, O Stuve… - The Journal of …, 2001 - Am Soc Clin Investig
AJ Slavin, JM Soos, O Stuve, JC Patarroyo, HL Weiner, A Fontana, EK Bikoff, SS Zamvil
The Journal of Clinical Investigation, 2001Am Soc Clin Investig
The role of processing in antigen (Ag) presentation and T cell activation in experimental
allergic encephalomyelitis (EAE) was evaluated in wild-type mice, mice that selectively
express either Ii p31 or p41, and mice completely deficient in Ii or H-2M. We demonstrate
that processing of myelin oligodendrocyte glycoprotein (MOG) is required for presentation of
the dominant encephalitogenic MOG epitope, p35-55. Ii p31-and p41-expressing mice
developed EAE with similar incidence to wild-type mice, although p41 mice had a more …
The role of processing in antigen (Ag) presentation and T cell activation in experimental allergic encephalomyelitis (EAE) was evaluated in wild-type mice, mice that selectively express either Ii p31 or p41, and mice completely deficient in Ii or H-2M. We demonstrate that processing of myelin oligodendrocyte glycoprotein (MOG) is required for presentation of the dominant encephalitogenic MOG epitope, p35-55. Ii p31- and p41-expressing mice developed EAE with similar incidence to wild-type mice, although p41 mice had a more severe course. Ag-presenting cells (APCs) from Ii- or H-2M–deficient mice could present p35-55, but not MOG, demonstrating that these APCs could not process native MOG. Ii- and H-2M–deficient mice were not susceptible to EAE by immunization with p35-55 or MOG or by adoptive transfer of encephalitogenic T cells. However, CD4+ T cells from p35-55–immunized H-2M–deficient mice proliferated, secreted IFN-γ, and transferred EAE to wild-type, but not H-2M–deficient, mice. Thus, EAE resistance in H-2M–deficient mice is not due to an inability of APCs to present p35-55, or an intrinsic defect in the encephalitogenic T cell repertoire, but reflects a defect in APC function. Our results indicate that processing is required for initial Ag presentation and CNS T cell activation and suggest that autopathogenic peptides of CNS autoantigen may not be readily available for presentation without processing.
The Journal of Clinical Investigation