RANK‐independent osteoclast formation and bone erosion in inflammatory arthritis

W O'Brien, BM Fissel, Y Maeda, J Yan… - Arthritis & …, 2016 - Wiley Online Library
W O'Brien, BM Fissel, Y Maeda, J Yan, X Ge, EM Gravallese, AO Aliprantis, JF Charles
Arthritis & rheumatology, 2016Wiley Online Library
Objective Proinflammatory molecules promote osteoclast‐mediated bone erosion by up‐
regulating local RANKL production. However, recent evidence suggests that combinations
of cytokines, such as tumor necrosis factor (TNF) plus interleukin‐6 (IL‐6), induce RANKL‐
independent osteoclastogenesis. The purpose of this study was to better understand TNF/IL‐
6–induced osteoclast formation and to determine whether RANK is absolutely required for
osteoclastogenesis and bone erosion in murine inflammatory arthritis. Methods Myeloid …
Objective
Proinflammatory molecules promote osteoclast‐mediated bone erosion by up‐regulating local RANKL production. However, recent evidence suggests that combinations of cytokines, such as tumor necrosis factor (TNF) plus interleukin‐6 (IL‐6), induce RANKL‐independent osteoclastogenesis. The purpose of this study was to better understand TNF/IL‐6–induced osteoclast formation and to determine whether RANK is absolutely required for osteoclastogenesis and bone erosion in murine inflammatory arthritis.
Methods
Myeloid precursors from wild‐type (WT) mice or mice with either germline or conditional deletion of Rank, Nfatc1, Dap12, or Fcrg were treated with either RANKL or TNF plus IL‐6. Osteoprotegerin, anti–IL‐6 receptor (anti–IL‐6R), and hydroxyurea were used to block RANKL, the IL‐6R, and cell proliferation, respectively. Clinical scoring, histologic assessment, micro–computed tomography, and quantitative polymerase chain reaction (qPCR) were used to evaluate K/BxN serum–transfer arthritis in WT and RANK‐deleted mice. Loss of Rank was verified by qPCR and by osteoclast cultures.
Results
TNF/IL‐6 generated osteoclasts in vitro that resorbed mineralized tissue through a pathway dependent on IL‐6R, NFATc1, DNAX‐activation protein 12, and cell proliferation, but independent of RANKL or RANK. Bone erosion and osteoclast formation were reduced, but not absent, in arthritic mice with inducible deficiency of RANK. TNF/IL‐6, but not RANKL, induced osteoclast formation in bone marrow and synovial cultures from animals deficient in Rank. Multiple IL‐6 family members (IL‐6, leukemia inhibitory factor, oncostatin M) were up‐regulated in the synovium of arthritic mice.
Conclusion
The persistence of bone erosion and synovial osteoclasts in Rank‐deficient mice, and the ability of TNF/IL‐6 to induce osteoclastogenesis, suggest that more than one cytokine pathway exists to generate these bone‐resorbing cells in inflamed joints.
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