[HTML][HTML] Beneficial effect of an omega-6 PUFA-rich diet in non-steroidal anti-inflammatory drug-induced mucosal damage in the murine small intestine
T Ueda, R Hokari, M Higashiyama… - World Journal of …, 2015 - ncbi.nlm.nih.gov
T Ueda, R Hokari, M Higashiyama, Y Yasutake, K Maruta, C Kurihara, K Tomita, S Komoto…
World Journal of Gastroenterology: WJG, 2015•ncbi.nlm.nih.govAIM: To investigate the effect of a fat rich diet on non-steroidal anti-inflammatory drug
(NSAID)-induced mucosal damage in the murine small intestine. METHODS: C57BL6 mice
were fed 4 types of diets with or without indomethacin. One group was fed standard
laboratory chow. The other groups were fed a fat diet consisting of 8% w/w fat, beef tallow
(rich in SFA), fish oil,(rich in omega-3 PUFA), or safflower oil (rich in omega-6 PUFA).
Indomethacin (3 mg/kg) was injected intraperitoneally from day 8 to day 10. On day 11 …
(NSAID)-induced mucosal damage in the murine small intestine. METHODS: C57BL6 mice
were fed 4 types of diets with or without indomethacin. One group was fed standard
laboratory chow. The other groups were fed a fat diet consisting of 8% w/w fat, beef tallow
(rich in SFA), fish oil,(rich in omega-3 PUFA), or safflower oil (rich in omega-6 PUFA).
Indomethacin (3 mg/kg) was injected intraperitoneally from day 8 to day 10. On day 11 …
Abstract
AIM: To investigate the effect of a fat rich diet on non-steroidal anti-inflammatory drug (NSAID)-induced mucosal damage in the murine small intestine.
METHODS: C57BL6 mice were fed 4 types of diets with or without indomethacin. One group was fed standard laboratory chow. The other groups were fed a fat diet consisting of 8% w/w fat, beef tallow (rich in SFA), fish oil,(rich in omega-3 PUFA), or safflower oil (rich in omega-6 PUFA). Indomethacin (3 mg/kg) was injected intraperitoneally from day 8 to day 10. On day 11, intestines and adhesions to submucosal microvessels were examined.
RESULTS: In the indomethacin-treated groups, mucosal damage was exacerbated by diets containing beef tallow and fish oil, and was accompanied by leukocyte infiltration (P< 0.05). The mucosal damage induced by indomethacin was significantly lower in mice fed the safflower oil diet than in mice fed the beef tallow or fish oil diet (P< 0.05). Indomethacin increased monocyte and platelet migration to the intestinal mucosa, whereas safflower oil significantly decreased monocyte and platelet recruitment (P< 0.05).
CONCLUSION: A diet rich in SFA and omega-3 PUFA exacerbated NSAID-induced small intestinal damage via increased leukocyte infiltration. Importantly, a diet rich in omega-6-PUFA did not aggravate inflammation as monocyte migration was blocked.
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