Systematic nucleo-cytoplasmic trafficking of proteins following exposure of MCF7 breast cancer cells to estradiol

G Pinto, AAM Alhaiek, S Amadi, AT Qattan… - Journal of proteome …, 2014 - ACS Publications
G Pinto, AAM Alhaiek, S Amadi, AT Qattan, M Crawford, M Radulovic
Journal of proteome research, 2014ACS Publications
We have used a proteomics subcellular spatial razor approach to look at changes in total
protein abundance and in protein distribution between the nucleus and cytoplasm following
exposure of MCF7 breast cancer cells to estradiol. The dominant response of MCF7 cells to
estrogen stimulation involves dynamic changes in protein subcellular spatial distribution
rather than changes in total protein abundance. Of the 3604 quantitatively monitored
proteins, only about 2% show substantial changes in total abundance (> 2-fold), whereas …
We have used a proteomics subcellular spatial razor approach to look at changes in total protein abundance and in protein distribution between the nucleus and cytoplasm following exposure of MCF7 breast cancer cells to estradiol. The dominant response of MCF7 cells to estrogen stimulation involves dynamic changes in protein subcellular spatial distribution rather than changes in total protein abundance. Of the 3604 quantitatively monitored proteins, only about 2% show substantial changes in total abundance (>2-fold), whereas about 20% of the proteins show substantial changes in local abundance and/or redistribution of their subcellular location, with up to 16-fold changes in their local concentration in the nucleus or the cytoplasm. We propose that dynamic redistribution of the subcellular location of multiple proteins in response to stimuli is a fundamental characteristic of cells and suggest that perturbation of cellular spatial control may be an important feature of cancer.
ACS Publications