Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers

AQ Lam, BS Freedman, R Morizane… - Journal of the …, 2014 - journals.lww.com
Human pluripotent stem cells (hPSCs) can generate a diversity of cell types, but few
methods have been developed to derive cells of the kidney lineage. Here, we report a highly
efficient system for differentiating human embryonic stem cells and induced pluripotent stem
cells (referred to collectively as hPSCs) into cells expressing markers of the intermediate
mesoderm (IM) that subsequently form tubule-like structures. Treatment of hPSCs with the
glycogen synthase kinase-3β inhibitor CHIR99021 induced BRACHYURY+ MIXL1+ …